This is a knockout-validated antibody summary, based on the publication "A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: DOCK 8
Catalog number: sc-376911
Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 119-277 mapping near the N-terminus of human DOCK 8. Recommended for detection of DOCK 8 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.
Western blot
Peripheral blood T cell lysates from a healthy control and a DOCK8null patient and mouse splenic T cells from WT and Dock8–/– mice.
A) Representative immunoblot of the indicated proteins following TCR ligation in human T cells from a healthy donor (HC) and a DOCK8null patient. H) Representative immunoblot of the indicated protein after TCR ligation in splenic T cells from Dock8–/–, Dock8pri/pri, and WT mice. Please see Figure 4a,h in the article [1].
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