This is a knockout-validated antibody summary, based on the publication "The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. WB for the indicated MiDAC components from total cell lysates of WT, Dnttip1 KO1 and Dnttip1 KO2 mESCs. Actin is the loading control. From [1].

Mouse monoclonal IgG1

Company: Novus Biologicals

Antibody: DNTTIP1

Catalog number: NBP2-02507

Summary: Mouse monoclonal IgG1 against full length human recombinant protein of human DNTTIP1. Reacts with human and mouse. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry and immunohistochemistry (paraffin).

Western blot

WT, Dnttip1 KO mESCs.

5% dry milk in TBST buffer for 1 hr at RT with gentle rocking.

1:500 dilution in 5% dry milk in TBST buffer overnight at 4°C with gentle rocking.

Horseradish peroxidase coupled secondary IgG-specific antibodies in TBST Buffer for 1 hr at room temperature with gentle rocking.

Immobilon Crescendo Western HRP Substrate (Millipore, WBLUR0500) and imaged on an Odyssey Fc imaging system (LI-COR, Model: 2800).

The same clone (OTI1F4) is sold as Novus Biologicals NBP2-02507; OriGene TA501132, CF501132; Invitrogen MA5-25095.