This is a knockout-validated antibody summary, based on two publications "The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress α-synuclein aggregation" (discussed below) [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot depicting KO of DNAJB6 expression in α-syn-DsRed HEK293T clone 1 and 2. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: DNAJB6

Catalog number: ab198995

Summary: Rabbit monoclonal IgG against a recombinant fragment within human DNAJB6 (aa 1-100, N terminal). Reacts with mouse, rat and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

WT and DNAJB6-KO α-syn-DsRed HEK293T cells.

5% (w/v) dried skimmed milk in TBS (25 mM Tris-HCl pH 7.5, 150 mM NaCl).

RDye fluorescently labeled secondary antibodies (Li-Cor, UK).