This is a knockout-validated antibody summary, based on the publication "PTEN and DNA-PK determine sensitivity and recovery in response to WEE1 inhibition in human breast cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot analysis of DNA-PK expression in MDA-MB-231 scrambled control and isogenic DNA-PK knock-out cell lines (clones #1 and #2). GAPDH expression was used as loading control. From [1].

Mouse monoclonal IgG2b (kappa light chain)

Company: Santa Cruz

Antibody: DNA-PKCS

Catalog number: sc-390849

Summary: Mouse monoclonal IgG2b (kappa light chain) specific for an epitope mapping between amino acids 4100-4128 at the C-terminus of human DNA-PKCS. Recommended for detection of DNA-PKcs of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

Control and DNA-PK knock-out MDA-MB-231 cells.

5% nonfat milk or BSA in TBS-T.

Western Lightning Plus-ECL Substrate Kit according to the manufacturer’s instructions (PerkinElmer).