DNA Ligase IV antibody | knockout validation | Cell Signaling 14649

This is a knockout-validated antibody summary, based on the publication "CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

DNA Ligase IV antibody | knockout validation | Cell Signaling 14649 figure 1
Figure 1. Immunoblot validation of CRISPR-Cas9 generated knockout cell lines that were pre-validated by deep sequencing to bear all-allelic frame shift mutations (knockout (KO) A, B) or heterozygous mutations (−/+). Size marker bands are indicated in the first column; expected protein sizes are given in the last column. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: DNA Ligase IV

Catalog number: 14649

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Leu771 of human DNA ligase IV protein. Reacts with human protein by western blot.

Validation Method

Western blot

Sample

WT and DNA Ligase IV-KO HEK 293 cells. Cells were lysed in 50 ml of Laemmli buffer per 96-well.

Blocking agent

5% milk.

Primary incubation

1:1,000 dilution.

References
  1. Schmid-Burgk J, Höning K, Ebert T, Hornung V. CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016;7:12338 pubmed publisher