DNA Ligase III antibody | knockout validation | Santa Cruz sc-390922

This is a knockout-validated antibody summary, based on the publication "The role of host DNA ligases in hepadnavirus covalently closed circular DNA formation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

DNA Ligase III antibody | knockout validation | Santa Cruz sc-390922 figure 1
Figure 1. Western blot of the indicated protein in HepDG10 control and ligase LIG1 and LIG3 single knockout cells. β-actin served as loading control. From [1].
Antibody information

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: DNA Ligase III

Catalog number: sc-390922

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 181-480 mapping near the N-terminus of human DNA Ligase III. Reacts with human by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

Control and LIG3 KO HepDG10 cells.

References
  1. Long Q, Yan R, Hu J, Cai D, Mitra B, Kim E, et al. The role of host DNA ligases in hepadnavirus covalently closed circular DNA formation. PLoS Pathog. 2017;13:e1006784 pubmed publisher