This is a knockout-validated antibody summary, based on the publication "The role of host DNA ligases in hepadnavirus covalently closed circular DNA formation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of the indicated protein in HepDG10 control and ligase LIG1 and LIG3 single knockout cells. β-actin served as loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: DNA Ligase I

Catalog number: sc-271678

Summary: Mouse monoclonal IgG1 (kappa light chain) specific for an epitope mapping between amino acids 3-21 at the N-terminus of human DNA Ligase I. Reacts with mouse, rat and human by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including equine.

Western blot

Control and LIG1 KO HepDG10 cells.