DLP1 antibody | knockout validation | BD Biosciences 611112
DOI
//dx.doi.org/10.13070/ko.en.7.2238
Date
2017-04-08

This is a knockout-validated antibody summary, based on the publication "Endogenous Drp1 mediates mitochondrial autophagy and protects the heart against energy stress", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: DLP1

Catalog number: 611112

Summary: Mouse monoclonal IgG1 against rat DLP1 aa. 601-722. Reacts with rat, human, mouse, and dog. Suitable for western blot and immunofluorescence.

Validation Method

Western blot

Sample

Heart tissue from Drp1-CKO mice. Homogenates were prepared using RIPA buffer containing 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.1% SDS, 1% Igepal CA-630, and 0.5% sodium deoxycholate with protease inhibitors (Sigma, P8340) at a 1:400 dilution.

Note

Immunoblot analysis of cardiac Drp1 in Drp1-CKO and control (Ctr) mice. Alpha myosin heavy chain–MerCreMer (αMHC-MCM): Tg-αMHCMCM. (n=3 per group). Please see Figure 5a in the article [1].

References
  1. Ikeda Y, Shirakabe A, Maejima Y, Zhai P, Sciarretta S, Toli J, et al. Endogenous Drp1 mediates mitochondrial autophagy and protects the heart against energy stress. Circ Res. 2015;116:264-78 pubmed publisher