DLC-1 antibody | knockout validation | BD Biosciences 612020

This is a knockout-validated antibody summary, based on the publication "Regulation of white and brown adipocyte differentiation by RhoGAP DLC1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

DLC-1 antibody | knockout validation | BD Biosciences 612020 figure 1
Figure 1. Western blots showing the absence of DLC1 protein in Dlc1-KO MEFs. Calnexin was included as a loading control. From [1].
Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: DLC-1

Catalog number: 612020

Summary: Mouse monoclonal IgG1 against human DLC-1 aa. 47-249. Reacts with human by western blot. It is not recommended for immunofluorescence.

Validation Method

Western blot

Sample

Wildtype and Dlc1-KO MEFs. Cells were lysed in RIPA buffer containing protease inhibitors.

References
  1. Sim C, Kim S, Brunmeir R, Zhang Q, Li H, Dharmasegaran D, et al. Regulation of white and brown adipocyte differentiation by RhoGAP DLC1. PLoS ONE. 2017;12:e0174761 pubmed publisher