This is a knockout-validated antibody summary, based on the publication "Enhanced Histamine-Induced Itch in Diacylglycerol Kinase Iota Knockout Mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Protein immunohistochemical staining shows that DGKI protein is cytoplasmic in small-diameter (arrowheads) WT mouse DRG neurons and is absent in Dgki-/- mouse DRG neurons. Scale bars = 50 μm. From [1].

Mouse monoclonal IgG2A

Company: R&D Systems

Antibody: DGK-iota

Catalog number: MAB6435

Summary: Mouse monoclonal IgG2A against recombinant human DGK‑ iota (Ser925-Val1065 (predicted)). Reacts with human and mouse. Suitable for western blot.

Immunohistochemistry

WT and Dgki-/- mouse DRG tissue.

10% normal donkey serum (S30, EMD-Millipore, Burlington, Massachusetts) in TBST (NDS/TBST) for 1 h at room temperature (approximately 23°C).

1:300 dilution in NDS/TBST overnight at room temperature.

Blocked again with NDS/TBST for 30 min, followed by 1:500 goat anti-mouse IgG2a-Alexa 488 (A21131, Thermo Fisher Scientific) in NDS/TBST for 6 h at room temperature.

Imaging was performed on a Zeiss LSM 710 confocal laser scanning microscope.

The same clone (M38) is sold as Novus Biologicals NBP1-44861; LifeSpan Biosciences LS-C112420, LS-C112513, LS-C112546; Abnova MAB6435, MAB6494, MAB6495; Abcam ab79559; Invitrogen MA1-10290, MA1-10291, MA1-10292, A15753, A15781, 10630D; Exbio 11-558-C025, 11-558-C100, 12-558-C100, 1B-558-C025, 1B-558-C100, PB-558-T100, 1F-558-T025, 1F-558-T100, 1P-558-T025, 1P-558-T100, 1A-558-T025, 1A-558-T100, T4-558-T025, T4-558-T100.