This is a knockout-validated antibody summary, based on publications "GSDME mediates caspase-3-dependent pyroptosis in gastric cancer", as cited below [1], "Gasdermin E deficiency attenuates acute kidney injury by inhibiting pyroptosis and inflammation" for western blot knockout validation (figure 2a) [2], "Caspase 3/GSDME-dependent pyroptosis contributes to chemotherapy drug-induced nephrotoxicity" for western blot knockout validation (figure 4a) [3], "Zika virus causes placental pyroptosis and associated adverse fetal outcomes by activating GSDME" for western blot knockout validation (figure 1d) [4], and "GSDME deficiency leads to the aggravation of UVB-induced skin inflammation through enhancing recruitment and activation of neutrophils" for western blot knockout validation (figure 1b) [5]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Abcam

Antibody: DFNA5/GSDME

Catalog number: ab215191

Summary: Rabbit monoclonal IgG against a recombinant fragment within human DFNA5/GSDME (aa 1 to the C-terminus). Reacts with mouse, rat and human. Suitable for western blot, immunoprecipitation and flow cytometry.

Western blot

Wildtype and GSDME-KO SGC-7901 cell lines.

5% albumin from bovine serum in PBS for 1 h.

1:1,000 dilution overnight at 4 °C.

1:2,000 dilution secondary antibodies (Abcam, ab6721) for 1 h at room temperature.

Western blotting detection system (Bio-Rad, USA).

Immunoblot of GSDME and actin (loading control) in wildtype (WT) and GSDME knockout (GE-KO) SGC-7901 cell lines. Please see Figure 4a in the article [1].