This is a knockout-validated antibody summary, based on the publication "DEPTOR Is an in Vivo Tumor Suppressor That Inhibits Prostate Tumorigenesis via the Inactivation of mTORC1/2 Signals", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. DU145 cells were treated with various doses of Torin-1 for 12 h and then harvested for Western blotting using the indicated antibodies. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: DEPTOR/DEPDC6

Catalog number: 11816

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Leu113 of human DEPTOR/DEPDC6 protein. Reacts with human, rat and monkey. Suitable for western blot, immunoprecipitation and immunohistochemistry (paraffin).

Western blot

Control and DEPTOR-KO DU145 cells. Cells were lysed in lysis buffer with protease inhibitors and phosphatase inhibitors.