DDX4 antibody | knockout validation | Cell Signaling 8761

This is a knockout-validated antibody summary, based on the publication "Germline factor DDX4 functions in blood-derived cancer cell phenotypes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

DDX4 antibody | knockout validation | Cell Signaling 8761 figure 1
Figure 1. Immunoblot results showing reduced DDX4 protein expression in knockout cell lines (C1–C3) compared to control (SC). From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: DDX4

Catalog number: 8761

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the amino terminus of human DDX4 protein. Reacts with human and mouse. It is predicted to react based on 100% sequence homology with monkey. Suitable for western blot, immunoprecipitation and immunofluorescence (frozen).

Validation Method

Western blot

Sample

Control and DDX4 knockout IM-9 cell lines.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:5,000 dilution peroxidase-conjugated anti-rabbit (Life Technologies).

Detection

Chemiluminescence solution (1.25 mM luminol, 68 uM coumaric acid, 0.0093% hydrogen peroxide, and 0.1 M Tris pH 8.6) for 1–10 min, exposed to film, and developed.

References
  1. Schudrowitz N, Takagi S, Wessel G, Yajima M. Germline factor DDX4 functions in blood-derived cancer cell phenotypes. Cancer Sci. 2017;: pubmed publisher