This is a knockout-validated antibody summary, based on the publication "CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Company: Epitomics, now part of Abcam
Antibody: DDB1
Catalog number: 3821-1, this antibody is the same as Abcam ab109027
Summary: Rabbit monoclonal against a synthetic peptide corresponding to residues at the C-terminus of human DDB1. Reacts with mouse, rat, human. Suitable for immunocytochemistry, western blot, immunoprecipitation, and immunohistochemistry-paraffin. Unsuitable for flow cytometry.
Western blot
DDB1-deleted hepatocytes collected in vivo
1:10000 dilution
HRP-linked secondary antibody
Enhanced Chemiluminescence Detection Kit (Amersham)
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.