This is a knockout-validated antibody summary, based on the publication "CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Bethyl
Antibody: DDB1
Catalog number: IHC-0013401
Summary: Rabbit polyclonal raised against a peptide corresponding to aa. 1100 and C-term of human protein. Reacts with human and mouse protein, and is predicted to react with rat, X. laevis, bovine, dog, horse, rabbit, pig, and monkey (among others) due to 100% sequence identity. Suitable for immunohistochemistry-paraffin and immunofluorescence.
Immunohistochemistry
Oocytes from Ddb1fl/fl;Gdf9-Cre mice. Mouse ovaries were fixed overnight in 10% PBS-buffered formalin, dehydrated and embedded in paraffin. Ovary samples were serially sectioned (5 μm thick)
1:400 dilution at room temperature for 1 h
biotin-labelled secondary antibodies for 30 min
Vectastain ABC kits and DAB peroxidase substrate kits (Vector Laboratories, Burlingame, CA)
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
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