This is a knockout-validated antibody summary, based on the publication "CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal IgG
Company: Proteintech
Antibody: DCAF1
Catalog number: 11612-1-AP
Summary: Rabbit polyclonal IgG against VPRBP fusion protein. Reacts with human, mouse, and rat. Suitable for western blot, immunoprecipitation, flow cytometry, immunohistochemistry and ELISA. against VPRBP fusion protein. Reacts with human, mouse, and rat. Suitable for western blot, immunoprecipitation, flow cytometry, immunohistochemistry and ELISA.
Immunohistochemistry
Dcaf1fl/fl;Gdf9-Cre ovaries at PD21. Mouse ovaries were fixed overnight in 10% PBS-buffered formalin, dehydrated and embedded in paraffin. Ovary samples were serially sectioned (5 μm thick).
1:200 at room temperature for 1 h
biotin-labelled secondary antibodies for 30 min
Vectastain ABC kits and DAB peroxidase substrate kits (Vector Laboratories, Burlingame, CA).
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.