This is a knockout-validated antibody summary, based on the publication "DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Quantification of FOXC2-positive spermatogonia (green) in histological sections of control and Dazl cKO adult testes. SOX9 marks Sertoli cells (gray), DAZL is in red, and DAPI marks DNA (blue). Select tubules are outlined via dotted orange line. Cells enlarged within insets are highlighted by arrowheads. Insets show spermatogonia that are DAZL-positive and FOXC2-positive (control and mosaic cKO panels); DAZL-negative and FOXC2-positive (mosaic and DAZL-negative cKO panels). Scale bar = 20 um. From [1].

Mouse monoclonal IgG1

Company: BioRad

Antibody: DAZL

Catalog number: MCA2336

Summary: Mouse monoclonal IgG1 against a synthetic peptide corresponding to sequence within the C terminal domain of human DAZL. Reacts with human, mouse, rat and monkey. Suitable for western blot, immunofluorescence, and immunochemistry (paraffin).

Western blot

Control and Dazl cKO mouse testis.

10% donkey serum and 2% BSA in PBS.

1:100 dilution.

Fluorophore-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories).

Confocal microscopy (Zeiss LSM 710).

The same clone (3/11A) is sold as Bio-Rad MCA2336; LifeSpan Biosciences LS-C188293.