DARS2 antibody | knockout validation | Proteintech 13807-1-AP
DOI
//dx.doi.org/10.13070/ko.en.6.1854
Date
2016-11-25

This is a knockout-validated antibody summary, based on the publication "Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

DARS2 antibody | knockout validation | Proteintech 13807-1-AP figure 1
Figure 1. Western blot of heart lysates from wild-type and DARS2 knockout mice (and from Clpp / DARS2 double knockout mice). Blot probed with Ant-DARS2 antibody. CTLR represents a loading control. From figure 4 in [1].
Antibody information

Rabbit polyclonal

Company: Proteintech

Antibody: DARS2

Catalog number: 13807-1-AP

Summary: Rabbit polyclonal antibody raised against a human DARS2 fusion protein. Supplier recommended for WB, IHC and elisa. The antibody is antigen affinity-purified. Reacts with human, mouse and rat DARS2.

Validation Method

Western blot

Sample

Heart extracts from wild-type and DARS2 knockout mice.

Primary incubation

Anti-DARS2 antibody diluted 1:200.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Seiferling D, Szczepanowska K, Becker C, Senft K, Hermans S, Maiti P, et al. Loss of CLPP alleviates mitochondrial cardiomyopathy without affecting the mammalian UPRmt. EMBO Rep. 2016;: pubmed