This is a knockout-validated antibody summary, based on the publication "Activation of IGF1R by DARPP-32 Promotes STAT3 Signaling in Gastric Cancer Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative immune-histochemical staining of DARPP-32 in gastric tissues from wild-type and DP KO 2–4 month old mice. From [1] and Courtesy from the Authors (IHC).

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: DARPP-32

Catalog number: sc-398144

Summary: Mouse monoclonal IgG1 (kappa light chain) against an epitope corresponding to amino acids 134-195 of bovine DARPP-32. Recommended for detection of DARPP-32 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

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Figure 2. Representative immune-histochemical fluorescent staining of DARPP-32 and p-STAT3 in gastric tissues from wild-type, TFF1 KO, DP KO, and TFF1 KO/DP KO 2–4 month old mice. From [1] and Courtesy from the Authors (IHC).

Western blot | Immunocytochemistry | Immunohistochemistry

WB: gastric tissues from wild-type, TFF1 KO, DARPP-32 KO, and TFF1 KO/DARPP-32 KO mice. Cells were lysed with RIPA buffer complemented with protease and phosphatase inhibitors that were purchased from Santa Cruz.

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Figure 3. Representative Western blot of DARPP-32 and others in gastric tissues from wild-type, TFF1 KO, DP KO, and TFF1 KO/DP KO 2–4 month old mice. From [1] and Courtesy from the Authors (IHC).

IHC: gastric tissues from wild-type, TFF1 KO, DARPP-32 KO, and TFF1 KO/DARPP-32 KO mice. Formalin-fixed paraffin-embedded.

IF: gastric organoids from wild-type, TFF1 KO, DARPP-32 KO and TFF1 KO/DARPP-32 KO mice.

IF: 1:400 dilution overnight at 4°C.

IHC: primary antibody overnight at 4°C.

IF: 1:800 dilution Alexa Fluor goat anti-mouse IgG (Invitrogen) at room temperature for 2h.

IHC: 1 h incubation at room temperature.

IF: inverted laser-scanning LSM880-Airyscan (Zeiss, Thornwood, NY) confocal microscope.

IHC: horseradish peroxidase activity was identified using a Histostain Plus kit (Invitrogen), following supplier’s instructions.