This is a knockout-validated antibody summary, based on the publication "Evidence against dopamine D1/D2 receptor heteromers", as cited below [1]. The antibody was also validated in the article "Validation of dopamine receptor DRD1 and DRD2 antibodies using receptor deficient mice" for immunohistochemistry and western blot (see figure 1a, 2a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Monoclonal IgG2a
Company: Sigma-Aldrich (under exclusive license from Emory University)
Antibody: D1 dopamine receptor
Catalog number: D2994 (clone 1-1-F11 s.6)
Summary: raised against a recombinant fusion protein containing the C-terminal 97 amino acids from the human D1 dopamine receptor. It detects monkey, human and rat D1 dopamine receptor. Supplier recommended for immunohistochemistry and Western blotting.
Immunohistochemistry
Mouse brain (nucleus accumbens) fixed with 4% paraformaldehyde in 0.1M sodium phosphate, pH 7.5
Tris-buffered saline, pH 7.4 containing 10 fetal bovine serum and 0.5% Triton X-100 for 2 hours at room temperature
Incubated overnight at 4oC at a dilution of 1:200 - 1:500
Alexa 647-conjugated goat anti-rat (Invitrogen) at 1:1000 for 2 hours at room temperature
Olympus Fluoview FV1000 confocal laser scanning microscope
See figure 5 from the article [1].
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