This is a knockout-validated antibody summary, based on the publication "Cytochrome P450 oxidoreductase contributes to phospholipid peroxidation in ferroptosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: Cytochrome P450 Reductase
Catalog number: ab180597
Summary: Rabbit monoclonal IgG against a synthetic peptide within human Cytochrome P450 Reductase (aa 100-200). Reacts with mouse, rat and human. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence and flow cytometry.
Western blot
Wildtype and POR-KO 786-O-Cas9 cells.
50% Odyssey blocking buffer (LiCor) diluted with 0.1% Tween-20-containing TBS (TBST).
1:1,000 dilution.
IRDye 800CW goat anti-Rabbit.
Immunoblotting images were acquired on an Odyssey equipment (LiCor).
Immunoblot analysis of POR protein levels in wildtype 786-O-Cas9 cells, and POR−/− single cell progenies (SCP) 1-4. β-Actin was used as a loading control. Please see Figure 2c in the article [1].
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