This is a knockout-validated antibody summary, based on the publication "The E3 ligase Itch and deubiquitinase Cyld act together to regulate Tak1 and inflammation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: Cell Signaling

Antibody: Cyld

Catalog number: 4495

Summary: Rabbit polyclonal against a synthetic peptide corresponding to residues surrounding His334 of human CYLD protein. Reacts with human, mouse and monkey protein and it is suitable for western blot and immunoprecipitation.

Western blot

WT, Itch−/− and Cyld−/− murine bone marrow derived macrophages (BMDMs). Cell lysis was performed using NP-40 buffer containing 50 mM Tris, pH8, 150 mM NaCl, 1% NP-40, EDTA-free protease inhibitor cocktail (Roche), 2 mM sodium fluoride (Boston Bioproducts), 10 mM-glycerophosphate (Boston Bioproducts), 1 mM sodium orthovanadate (Sigma).

Amersham ECL or ECL plus western blotting detection system (GE).

Please see Figure 5a in the article [1].

Authors show validation with mouse protein, that company doesn’t say it is detected. Probably a mistake.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.