This is a knockout-validated antibody summary, based on the publication "Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: Cyclin D2
Catalog number: 3741
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the amino terminus of cyclin D2. Reacts with human, mouse and rat. Suitable for western blot and immunoprecipitation.
Western blot
Wild type and combinational (cyclin D1−/−D2−/−D3−/−) or single isoform cyclin D knockout MEFs. Cells were lysed in EBC buffer (50 mM Tris pH 7.5, 120 mM NaCl, 0.5% NP-40) supplemented with protease inhibitors (Complete Mini, Roche) and phosphatase inhibitors (phosphatase inhibitor cocktail set I and II, Calbiochem).
Peroxidase-conjugated anti-rabbit secondary antibody (Sigma, A-4914).
Immunoblot analysis of whole cell lysates derived from wild type versus combinational (cyclin D1−/−D2−/−D3−/−) (a) or single isoform cyclin D knockout MEFs. Please see Figure 2a,b in the article [1].
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