This is a knockout-validated antibody summary, based on the publication "Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: Cyclin D1
Catalog number: 2978
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the carboxy-terminus of human cyclin D1. Reacts with human, mouse and rat. Suitable for western blot, immunohistochemistry (paraffin) and flow cytometry.
Western blot
Wild type and combinational (cyclin D1−/−D2−/−D3−/−) or single isoform cyclin D knockout MEFs. Cells were lysed in EBC buffer (50 mM Tris pH 7.5, 120 mM NaCl, 0.5% NP-40) supplemented with protease inhibitors (Complete Mini, Roche) and phosphatase inhibitors (phosphatase inhibitor cocktail set I and II, Calbiochem).
Peroxidase-conjugated anti-rabbit secondary antibody (Sigma, A-4914).
Immunoblot analysis of whole cell lysates derived from wild type versus combinational (cyclin D1−/−D2−/−D3−/−) (a) or single isoform cyclin D knockout MEFs. Please see Figure 2a,b in the article [1].
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