This is a knockout-validated antibody summary, based on the publication "Gap junction communication between uterine stromal cells plays a critical role in pregnancy-associated neovascularization and embryo survival", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal IgG

Company: Zymed

Antibody: Cx43

Catalog number: 71-0700

Summary: Rabbit polyclonal IgG against a peptide corresponding to a segment of the 3rd cytoplasmic domain (C-terminal portion) of rat connexin 43. Reacts with human, mouse, rat, and bovine. Suitable for western blot, immunofluorescence, immunohistochemistry and ELISA.

Immunohistochemistry

Uterine sections from Cx43fl/fl (control) and Cx43d/d (conditional knockout) mice. Tissues were obtained and flash frozen, embedded in OCT, and cryosections were taken at 8 µm.

1:500 dilution overnight at 4°C.

1:200 dilution of TRITC-conjugated goat anti-rabbit antibody (Sigma T6778) for 2 hours at room temperature.

Please see Figure 2 in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.