This is a knockout-validated antibody summary, based on the publication "Attenuated Levels of Hippocampal Connexin 43 and its Phosphorylation Correlate with Antidepressant- and Anxiolytic-Like Activities in Mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis of Cx43 levels in the hippocampus of Cx43fl/fl mice relative to control Cx43wt/wt mice. β-actin was used as a loading control. Figure 1 [1].

Antibody: Cx43

Catalog number: 610062

Summary: Mouse IgG1 raised against rat connexin-43 peptide (aa. 252-270).It reacts with rat, human, mouse, dog, and chicken proteins. It has been successfully used for western blot, immunofluorescence,and immunohistochemistry-formalin (antigen retrieval required).

Company: BD Biosciences

Western blot, WB

7 week-old male transgenic Cx43fl/fl mice frozen hippocampi were pulverized on dry ice, resuspended in boiling 2% SDS containing protease inhibitors (Roche), β-glycerophosphate (10 mM) and orthovanadate (1 mM), and sonicated on ice. Lysates were centrifugated at 13,000 rpm at 4°C and supernatants were diluted with 5x Laemmli buffer, and boiled 5 min.

Membranes were blocked for 1 h with 5% fat-free dried milk/TBS1x-0.1% Tween

incubated overnight at 4°C, 1:500 dilution

goat anti-mouse (HRP-IgG, Santa Cruz, sc-2005, 1/2500), for 2 h at room temperature.

Chemiluminescent signal was obtained using Western Lighting Plus ECL detection kit (Perkin-Elmer NEL104001EA).