This is a knockout-validated antibody summary, based on the two publications "Expression of connexins 36, 43, and 45 during postnatal development of the mouse retina" [1], and "Prolonged dark adaptation changes connexin expression in the mouse retina" [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: Zymed (Thermo Fisher Scientific)

Antibody: Cx36

Catalog number: 36-4600

Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the C-terminal region of human connexin 36. Supplier recommended for WB, IP and IHC. Antibody is antigen affinity purified. Reacts with human, rat, mouse, Zebrafish and chicken connexin 36.

Immunohistochemistry

Mouse retinal sections fixed with 4% paraformaldehyde.

Phosphate buffer containing 3% goat serum, 1% BSA and 0.5% Triton X-100.

Anti-Cx36 antibody.

Alexa TM 488-conjugated goat anti-rabbit IgG (Molecular Probes).

Nikon PCM2000 confocal microscope in conjunction with Adobe Photoshop 7.0 software.

See figure 2 in [1].

Immunohistochemistry

Eyes from wild-type and connexin 43 -/- mice fixed with 4% paraformaldehyde.

PBS containing 3%goat serum, 1% BSA and 0.5% Triton X-100.

Anti-connexin 43 antibody (1μg/ml) in PBS containing 3% goat serum and 0.5% Triton X-100.

Alexa TM-conjugated anti-rabbit IgG (1:1000, Molecular Probes).

Nikon PCM2000 confocal microscope.

See supplemental figure 4 in [2].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.