This is a knockout-validated antibody summary, based on the publication "Tissue-specific cross-reactivity of connexin32 antibodies: problems and solutions unique to the central nervous system", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a

Company: Zymed

Antibody: Cx32

Catalog number: 35-8900

Summary: Mouse monoclonal IgG2a against a synthetic peptide derived from the C-terminus of mouse Connexin 32. Reacts with rat and mouse. Suitable for for western blot, immunofluorescence and immunohistochemistry (frozen).

Western blot

Brain and liver tissues from human and WT, and Cx32 knockout mice. All tissues were homogenized in fresh RIPA buffer (1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM NaF, 50 g/ml aprotinin, 1 mM sodium orthovanadate, 1 mg/ml phenylmethylsulfonyl fluoride, 10 mM phosphate-buffered saline (PBS; 10 mM phosphate, 154 mM NaCl)).

5% (w/v) skim milk powder–PBS with 0.1% Tween-20 (PBST; blocking buffer) for 1 to 3 h.

3.0 μg/ml in blocking buffer overnight at 4°C.

Horseradish peroxidase (HRP)-conjugated anti-mouse (Jackson ImmunoResearch

Laboratories, PA; 1:2,000) diluted in blocking buffer for 1- to 3-h.

X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Pierce, IL).

Please see Figure 3e in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.