This is a knockout-validated antibody summary, based on the publication "Cx26 knockout predisposes the mammary gland to primary mammary tumors in a DMBA-induced mouse model of breast cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunofluorescence revealed considerable but not complete (insert) knockout of Cx26(green/red, arrows) in the mammary gland of BLG-Cre; Cx26fl/fl mice (Cre+), unlike Cx26fl/fl control mice (Cre-) of both mice treated with DMBA 1 and 12 weeks after pituitary transplant. Hoechst denotes nuclei. Scale bars = 50 µm. N≥6. From [1].

Rabbit polyclonal

Company: Invitrogen

Antibody: Cx26

Catalog number: 51-2800

Summary: Rabbit polyclonal against a 13 amino acid synthetic peptide derived from the C-terminus of the mouse Connexin 26 protein. Reacts with rat, mouse, and human in western blot, immunofluorescence/immunocytochemistry, immunohistochemistry and ELISA.

Immunohistochemistry

Cryosections of primary mammary glands from conditional knockout mouse where the Cre transgene was under the control of the β-lactoglobulin (BLG) promoter.

3% BSA in PBS with 0.02% Triton X-100.

1:100 dilution overnight at 4°C.

1:400 dilution of Alexa Fluor® 488 or 555-conjugated anti-rabbit (Molecular Probes, A21429/A11008) for 1 hour at room temperature.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.