This is a knockout-validated antibody summary, based on the publication "Cx26 knockout predisposes the mammary gland to primary mammary tumors in a DMBA-induced mouse model of breast cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Representative images of paraffin-embedded primary mammary tumor sections immunolabelled with Cx26 (i, ii, iii, red). Hoechst denotes nuclei. Scale bars=50 μm. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Invitrogen

Antibody: Cx26

Catalog number: 13-8100

Summary: Mouse monoclonal IgG1 (kappa light chain) against a synthetic peptide corresponding to a segment of the cytoplasmic loop of rat connexin-26. Reacts with rat, mouse, and human in western blot, immunofluorescence, immunohistochemistry (frozen) and ELISA.

Immunohistochemistry

Paraffin-embedded primary mammary tumor sections from conditional knockout mouse where the Cre transgene was under the control of the β-lactoglobulin (BLG) promoter.

3% BSA in PBS with 0.02% Triton X-100.

1:100 dilution overnight at 4°C.

1:400 dilution Alexa Fluor® 488 or 555-conjugated anti-mouse (Molecular Probes, A21425/A11017) for 1 hour at room temperature.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.