This is a knockout-validated antibody summary, based on the publication "Drug transporters on arachnoid barrier cells contribute to the blood-cerebrospinal fluid barrier", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunochemical staining of P-gp by rabbit polyclonal custom-made P-gp antibody in arachnoid barrier cells (solid arrowhead) and in blood vessel endothelial cells of the blood-brain barrier in the brain, of P-gp WT mice (A) and P-gp KO mice (B), from figure 2 [1].

Rabbit polyclonal, against amino acids 555–575 of human P-gp and immunopurified with the same peptide.

Antibody: P-gp (the one validated was produced in lab with rabbits)

Catalog number: JSB-1 (ab3366)

Summary: Mouse monoclonal to P Glycoprotein. specific for the MDR1 isoform of P Glycoprotein which is only found in the plasma membrane. It does not cross-react with MDR3. It has been shown to cross-react with Pyruvate Carboxylase, an abundant mitochondrial enzyme (130 kDa), on both immunoblots and immunohistochemical tissue sections. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P glycoprotein. Reacts with mouse, rat, and human and has been used for western blot, ELISA, immunocytochemistry, immunohistochemistry (paraffin), immunoprecipitation and flow cytometry.

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Figure 2. Immunochemical staining of P-gp by rabbit polyclonal custom-made P-gp antibody in arachnoid barrier cells (solid arrowhead) and in blood vessel endothelial cells of the blood-brain barrier in the spine of P-gp WT mice (C) and P-gp KO mice (E), from figure 2 [1].

IHC, immunohistochemistry

Female P-glycoprotein (P-gp) knockout (KO) CF-1 mice. Tissue was fixed with 10% formalin for 24 hours and paraffin sections processed by heat-induced epitope retrieval.

Slides were blocked with 5% normal goat serum/phosphate-buffered saline (PBS) for 30 minutes.

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Figure 3. Immunochemical staining of P-gp by rabbit polyclonal custom-made P-gp antibody in WT (left) and P-gp KO (right) mice. Courtesy of the authors.

Overnight incubation with rabbit anti-P-gp (1:4000) or with Abcam mouse anti-P-gp antibody JSB-1 (1:20).

Detected with the streptavidin-biotin immunoperoxidase method and diaminobenzidine substrate for visualization.

Both rabbit P-gp antibody made by the researchers and mouse (JSB-1, ab3366) antibody show similar staining patterns and both are considered validated.

The same clone (JSB-1) is sold as Bio-Rad MCA857H; BioLegend 901401; Cell Sciences MON9011-1; LifeSpan Biosciences LS-C11667, LS-C85155, LS-C122115, LS-B5570, LS-C312100; Abcam ab3366; Invitrogen MA1-26530, MA1-35604, 180160Z; EMD Millipore MAB4120.