Cul3 antibody | knockout validation | Bethyl Laboratories A301-109A
DOI
//dx.doi.org/10.13070/ko.en.6.1837
Date
2016-11-20

This is a knockout-validated antibody summary, based on the publication "Hypertension-causing Mutations in Cullin3 Protein Impair RhoA Protein Ubiquitination and Augment the Association with Substrate Adaptors", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Cul3 antibody | knockout validation | Bethyl Laboratories A301-109A figure 1
Figure 1. Immunoblot of Cul3 in HEK293T and HEK293TCul3KO cells. GAPDH served as loading control. The position of each molecular weight size marker (in kilodaltons) is shown. From [1].
Antibody information

Rabbit polyclonal IgG

Company: Bethyl Laboratories

Antibody: Cul3

Catalog number: A301-109A

Summary: Rabbit polyclonal IgG against an epitope between aa. 650 and 700 of human cullin 3. Reacts with human and mouse protein in western blot, immunoprecipitation, immunohistochemistry (paraffin) and immunofluorescence.

Validation Method

Western blot

Sample

HEK293T and CRISPR/Cas9-mediated HEK293TCul3KO.

Detection

ECL (Amersham Biosciences).

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Ibeawuchi S, Agbor L, Quelle F, Sigmund C. Hypertension-causing Mutations in Cullin3 Protein Impair RhoA Protein Ubiquitination and Augment the Association with Substrate Adaptors. J Biol Chem. 2015;290:19208-17 pubmed publisher