This is a knockout-validated antibody summary, based on the publication "The presynaptic ribbon maintains vesicle populations at the hair cell afferent fiber synapse", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Maximum intensity projections of confocal z-sections of inner hair cells (IHCs) in apical cochlea turns of P22 KO and wildtype littermates, immunolabeled for CtBP2/RIBEYE (A), Homer (B), Bassoon (C) and Homer-Bassoon Overlay (D). Virtually no punctated extra-nuclear CtBP2 staining that is colocalized with other synaptic proteins can be seen in KO mice, whereas Bassoon and Homer appear generally unaffected. From [1].

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: CtBP2

Catalog number: 612044

Summary: Mouse monoclonal IgG1 against ouse CtBP2 (aa. 361-445). Reacts with mouse, human, rat and dog. Suitable for western blot and immunofluorescence.

Immunohistochemistry

Cochlea from wildtype and CtBP2 KO mice.

4% (w/v) BSA/PBS at room temperature for 2 hr.

1:400 dilution in blocking buffer overnight at 4˚C.

1:600 dilution anti-mouse secondary antibodies (Alexa Fluor 488, 546 or 555, 647) in 4% [w/v] BSA/PBS) for 1 hr at room temperature.

Confocal microscope (LSM700 or LSM880, Zeiss) using 1.4 NA oil immersion objectives for confocal imaging, and using 1.2 NA water immersion objective for Airyscan imaging.