This is a knockout-validated antibody summary, based on the publication "Association of connexin36 with zonula occludens-1 in HeLa cells, betaTC-3 cells, pancreas, and adrenal gland", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: Zymed (ThermoFisher Scientific)

Antibody: Connexin 36

Catalog number: 51-6300

Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the C-terminal region of rat and mouse connexin 36.

Supplier recommended for WB, IP and elisa.

Antigen affinity-purified.

Reacts with human, mouse and rat connexin 36.

Western blot

Lysates of pancreas from wild-type and connexin 36 knockout mice.

TBS containing 0.2% Tween 20 and 5% milk powder.

Anti-connexin 36 antibody diluted to 1μg/ml in TBS containing 0.2 Tween 20 and 1% milk powder.

HRP-conjugated anti-rabbit IgG diluted 1:5000.

ECL reagent (GE Healthcare).

See Figure 1E in [1].

This paper also indicates that this antibody has been KO validated by immunohistochemistry. However, the raw data was not published.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.