This is a knockout-validated antibody summary, based on the publication "Association of connexin36 with zonula occludens-1 in HeLa cells, betaTC-3 cells, pancreas, and adrenal gland", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal

Company: Zymed (ThermoFisher Scientific)

Antibody: Connexin 36

Catalog number: 37-4600

Summary: Mouse monoclonal antibody raised against a peptide corresponding to the C-terminal region of rat and mouse connexin 36.

Supplier recommended for IF and ICC. Supplier also reports use in WB, IP and IHC.

Reacts with human and mouse connexin 36.

Immunohistochemistry

Sections of pancreas from wild-type and connexion 36 knockout mice.

TBS containing 0.2% Tween 20 and 10% normal goat serum.

Anti-connexin 36 antibody diluted to 3μg/ml.

AlexaFluor 488-conjugated goat anti-mouse IgG diluted 1:1000 (Molecular Probes).

Zeiss Axioscope 2 fluorescent microscope with Zeiss Axiovision 3.0 image software.

The authors describe in the text data which appears to support the KO validation of this antibody by immunohistochemistry. However, the raw data was not published.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.