This is a knockout-validated antibody summary, based on the publication "Association of connexin36 with zonula occludens-1 in HeLa cells, betaTC-3 cells, pancreas, and adrenal gland", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: Zymed (ThermoFisher Scientific)

Antibody: Connexin 36

Catalog number: 36-4600

Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the C-terminal region of human connexin 36.

Supplier recommended for WB, IHC and elisa.

Antigen affinity-purified.

Reacts with human and mouse connexin 36.

Western blot

Immunohistochemistry

Western blot: Lysates of adrenal gland from wild-type and connexin 36 knockout mice.

Immunohistochemistry: sections of pancreas from wild-type and connexion 36 knockout mice.

Western blot: TBS containing 0.2% Tween 20 and 5% milk powder.

Immunohistochemistry: TBS containing 0.2% Tween 20 and 10% normal goat serum.

Western blot: anti-connexin 36 antibody diluted to 1μg/ml in TBS containing 0.2 Tween 20 and 1% milk powder.

Immunohistochemistry: anti-connexin 36 antibody diluted to 3μg/ml.

Western blot: HRP-conjugated anti-rabbit IgG diluted 1:5000.

Immunohistochemistry: AlexaFluor 488-conjugated goat anti-rabbit IgG diluted 1:1000 (Molecular Probes).

Western blot: ECL reagent (GE Healthcare).

Immunohistochemistry: Zeiss Axioscope 2 fluorescent microscope with Zeiss Axiovision 3.0 image software.

Figure 1D (Western blot) and Figure 2 (immunohistochemistry) in [1].

This paper also reports on the use of two other connexin 36 antibodies from the same manufacturer (Catalog numbers 37-4600 and 51-6300, respectively). The authors indicate that 37-4600 and 51-6300 gave essentially the same immunohistochemistry results as 36-4600 (shown here), but the raw data was not published.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.