This is a knockout-validated antibody summary, based on the publication "Minimized human telomerase maintains telomeres and resolves endogenous roles of H/ACA proteins, TCAB1, and Cajal bodies", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b
Company: Abcam
Antibody: Coilin
Catalog number: ab87913
Summary: Mouse monoclonal IgG2b against a synthetic peptide of human Coilin. Reacts with human protein by and monkey by western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.
Western blot | Immunocytochemistry
TCAB1 and Coilin in clonal KO HCT116 cells (with LhTRmin + TERT overexpression).
WB: 4% nonfat milk (Carnation) in TBS buffer (150 mM NaCl, 50 mM Tris pH 7.5).
ICC: 4% BSA in PBS for 1 hr at room temperature or overnight at 4°C.
WB: 1:250 dilution in blocking buffer at 4°C overnight.
ICC: 1:250 in blocking buffer for 1 hr at room temperature or overnight at 4°C.
WB: 1:2,000 dilution goat anti-mouse Alexa Fluor 680 (Life Technologies, Carlsbad, CA) in blocking buffer buffer for 1 hr at room temperature.
ICC: Alexa Fluor 488 (Thermo) secondary antibodies diluted in blocking buffer for 1 hr at room temperature.
WB: blots were scanned using a LI-COR Odyssey imager.
ICC: images were acquired using a Zeiss LSM510 Meta confocal microscope with a ×100/1.49 Apo objective, with 364-, 488-, and/or 543-nm laser excitation.
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
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