Claudin 2 antibody | knockout validation | ThermoFisher 32-5600

This is a knockout-validated antibody summary, based on the publication "Claudin-2 promotes colorectal cancer liver metastasis and is a biomarker of the replacement type growth pattern", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Claudin 2 antibody | knockout validation | ThermoFisher 32-5600 figure 1
Figure 1. Immunoblot analysis of Claudin-2 expression in parental HT-29 cells, HT-29 cells with Claudin-2 knockout (Cldn2KO) and HT-29 cells lacking endogenous Claudin-2 that were engineered to re-express Claudin-2 (Cldn2KO rescue). Total cell lysates were blotted for α-Tubulin as a loading control. From [1].
Antibody information

Mouse monoclonal IgG2b

Company: ThermoFisher

Antibody: Claudin 2

Catalog number: 32-5600

Summary: Mouse monoclonal antibody against a synthetic peptide corresponding to a 26 amino acid sequence at the C-terminus of mouse Claudin-2. Reacts with dog, human, mouse, and rat. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence, flow cytometry and ELISA.

Validation Method

Western blot

Sample

Parental and Claudin-2 knockout HT-29 cells.

Primary incubation

1:5,000 dilution.

Secondary incubation

Corresponding Horseradish-peroxidase-conjugated anti-IgG secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.)

Detection

Chemiluminescent HRP Substrate (Cat. #: WBLUF0500, Millipore) or an enhanced chemiluminescence system (Cat. #: 34578, Thermofisher).

References
  1. Tabariès S, Annis M, Lazaris A, Petrillo S, Huxham J, Abdellatif A, et al. Claudin-2 promotes colorectal cancer liver metastasis and is a biomarker of the replacement type growth pattern. Commun Biol. 2021;4:657 pubmed publisher