This is a knockout-validated antibody summary, based on the publication "Role of Clathrin Light Chains in Regulating Invadopodia Formation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of WT, CLCA−/−, CLCB−/− and CLCA−/− CLCB−/− cell lines using an antibody against CLC and actin. From [1].

Mouse monoclonal IgG2b

Company: Merck-Sigma Aldrich

Antibody: Clathrin Light Chain

Catalog number: C1985

Summary: Mouse monoclonal antibody against a synthetic peptide (amino acids 23-44 in bovine) common to the N-terminus of mammalian clathrin light chains, LCa and LCb. Reacts with human, mouse and rat. Suitable for western blot, immunocytochemistry, immunoprecipitation and microarray.

Western blot

WT, CLCA−/− and CLCB−/− U373 cell lines.

5% milk in TBST (50 mM Tris-HCL pH 7.5, 150 mM NaCl, 0.1% Tween) for 1 h at room temperature.

1:200 dilution in TBST with 5% milk overnight at 4 °C.

1:50,000 dilution secondary anti-mouse antibodies coupled to Horseradish Peroxidase (HRP) (GE Healthcare, Chicago, IL, USA, NA931) in TBST with 5% milk for 1 h at room temperature.

ECL Western Blotting detection reagents (GE Healthcare, RNP2106) or Western Bright Chemiluminescence Substrate Sirius (Biozym, 541021) using high performance chemiluminescence films (GE Healthcare, #28906837).

The same clone (CON.1) is sold as BioLegend 811501; MilliporeSigma C1985; Santa Cruz Biotechnology sc-12735; LifeSpan Biosciences LS-C87670; Invitrogen MA5-11860.