Chk2 antibody | knockout validation | Millipore 05-649

This is a knockout-validated antibody summary, based on the publication "Validating the concept of mutational signatures with isogenic cell models", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Chk2 antibody | knockout validation | Millipore 05-649 figure 1
Figure 1. Immunoblots for expression of the indicated proteins in CRISPR-Cas9 edited human HAP1 clones. Actin serves as a loading control. “*” denotes a non-specific band. From [1].
Antibody information

Mouse monoclonal IgG1

Company: Millipore

Antibody: Chk2

Catalog number: 05-649

Summary: Mouse monoclonal IgG1 against a mixture of two GST fusion proteins corresponding to residues 1-223 and 217-543 of human Chk2. Reacts with human and mouse. Suitable for western blot, immunoprecipitation and immunocytochemistry.

Validation Method

Western blot

Sample

Control and Chk2-KO human HAP1 cells. Cell extracts were prepared using RIPA lysis buffer (NEB) with protease (Sigma) and phosphatase (Sigma) inhibitors.

Primary incubation

1:1,000 dilution.

References
  1. Zou X, Owusu M, Harris R, Jackson S, Loizou J, Nik Zainal S. Validating the concept of mutational signatures with isogenic cell models. Nat Commun. 2018;9:1744 pubmed publisher