This is a knockout-validated antibody summary, based on the publication "Loss of PTEN Facilitates Rosiglitazone-Mediated Enhancement of Platinum(IV) Complex LA-12-Induced Apoptosis in Colon Cancer Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting of p53 protein in HCT116 wt and Chk2-/- cells. β-actin served as loading control. From [1].

Mouse monoclonal IgG2b

Company: Cell Signaling Technology

Antibody: Chk2

Catalog number: 3440

Summary: Mouse monoclonal IgG2b raised against truncated recombinant GST-Chk2. Reacts with human, monkey by western blotting, immunohistochemistry (paraffin), and immunofluorescence (immunocytochemistry).

Western blot

Human colon adenocarcinoma cell lines HCT116 wt (p53+/+, Bax+/-, Chk2+/+, PTEN+/+) and Chk2-/-. Cells were lysed in 1% SDS lysis buffer containing protease inhibitor cocktail (#P2714, Sigma–Aldrich) or Protease Inhibitor Cocktail Set I (#5391313, Calbiochem, Merck Millipore, Bedford, MA, USA), for phosphoprotein detection NaVO4 and NaF were added to the lysis solution.

5% non-fat milk or BSA for 1 h at RT.

1:1,000 dilution in 5% non-fat milk at 4°C overnight.

anti-mouse IgG (1:3,000, #NA931) (Amersham Biosciences, Piscataway, NJ, USA) for 1 h at RT.

Immobilon Western Chemiluminescent HRP Substrate (#WBKLS0500, Merck Millipore, Bedford, MA, USA).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.