This is a knockout-validated antibody summary, based on the publication "Specific role of Chk1 phosphorylations in cell survival and checkpoint activation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal
Company: Santa Cruz Biotechnology
Antibody: Chk1 (Clone G-4)
Catalog number: sc-8408
Summary: Mouse monoclonal raised against residues 1-476 of human Chk1.
Supplier recommended for WB, IP, IF, IHC, flow cytometry and elisa.
Reacts with mouse, rat and human Chk1.
Western blot
Lysates of Chk1lox/− embryonic stem cells transduced with adenoviruses expressing Chk1 and Cre recombinase to knockout endogenous Chk1 expression.
Anti-Chk1 antibody.
see Figure 3 from [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- Niida H, Katsuno Y, Banerjee B, Hande M, Nakanishi M. Specific role of Chk1 phosphorylations in cell survival and checkpoint activation. Mol Cell Biol. 2007;27:2572-81 pubmed
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
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