Cell Signaling Technology rabbit monoclonal antibody 8540 was validated in knockout cell line

DOI

http://dx.doi.org/10.13070/ko.en.6.1570

Date

2016-08-23

This is a knockout-validated antibody summary, based on the publication "Golgi-Resident GTPase Rab30 Promotes the Biogenesis of Pathogen-Containing Autophagosomes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of wild type and Atg5-KO HeLa cells. Beta-actin was used as loading control.

Antibody

Atg5, also called APG5; APG5-LIKE; APG5L; ASP; hAPG5; ATG5 autophagy related 5 homolog; apoptosis-specific protein.

Catalog number

Catalog number is 8540. This product has been discontined by CST.

Summary

Rabbit monoclonal antibody that recognizes endogenous levels of total Atg5 protein. Animals were immunized with a synthetic peptide corresponding to residues surrounding Pro187 of human Atg5 protein.

Company

Cell Signaling Technology

Validation Method

Western blot

Sample

Atg5-KO HeLa cells

Secondary incubation

horseradish peroxidase-conjugated anti-rabbit (Jackson Immunoresearch Laboratories).

References

Oda S, Nozawa T, Nozawa-Minowa A, Tanaka M, Aikawa C, Harada H, et al. Golgi-Resident GTPase Rab30 Promotes the Biogenesis of Pathogen-Containing Autophagosomes. PLoS ONE. 2016;11:e0147061 pubmed publisher

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