This is a knockout-validated antibody summary, based on the publication "H2AX deficiency is associated with erythroid dysplasia and compromised haematopoietic stem cell function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot for histone H2AX in wild type (WT), H2AX heterozygous (Het) and H2AX null (KO) mouse bone marrow cells. Hsc70 is used as loading control. Courtesy of the authors.

Antibody

H2AX, H2A histone family member X.

Catalog number is 2595.

Rabbit polyclonal antibody that detects endogenous levels of histone H2A.X protein from Human, Mouse, Rat, Monkey origin. Applied successfully in Western blot.

Cell Signaling

Western blot

h2; Sample

H2AX knockout mice bone marrow cells

H2AX knockout mice exhibited macrocytic anaemia, cell-autonomous anaemia and erythroid dysplasia, mimicking dyserythropoiesis in myelodysplastic syndromes.