Cdk5 antibody | knockout validation | Santa Cruz sc-249

This is a knockout-validated antibody summary, based on the publication "Cdk5-dependent phosphorylation of liprinα1 mediates neuronal activity-dependent synapse development", as cited below [1], and "CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity" for western blot knockout validation (figure 1f) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Cdk5 antibody | knockout validation | Santa Cruz sc-249 figure 1
Figure 1. Western blot of Cdk5 in brains from Cdk5+/+ and Cdk5−/− E18 mice. Courtesy from the Authors [1].
Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: Cdk5

Catalog number: sc-249

Summary: Mouse monoclonal antibody against recombinant full length human Cdk5. Recommended for detection of Cdk5 of mouse, rat, human and Drosophila origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

Brains from Cdk5+/+ and Cdk5−/− E18 mice.

Primary incubation

1:5,000 dilution.

References
  1. Huang H, Lin X, Liang Z, Zhao T, Du S, Loy M, et al. Cdk5-dependent phosphorylation of liprin?1 mediates neuronal activity-dependent synapse development. Proc Natl Acad Sci U S A. 2017;114:E6992-E7001 pubmed publisher
  2. Sang Y, Li Y, Zhang Y, Alvarez A, Yu B, Zhang W, et al. CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity. Nat Commun. 2019;10:4013 pubmed publisher