Cdk2 antibody | knockout validation | Abcam ab32147

This is a knockout-validated antibody summary, based on the publication "PolyC-binding proteins enhance expression of the CDK2 cell cycle regulatory protein via alternative splicing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Cdk2 antibody | knockout validation | Abcam ab32147 figure 1
Figure 1. Western blot analysis of CDK2 ex5(–) K562 clones. The identity of each cell clone is shown above the corresponding lane. The positions of the full-length CDK2 (CDK2 FL) protein, the CDK2 ex5(–), and the loading control L7a are indicated. CDK2#1 and CDK2#2 are two distinct CDK2 primary antibodies. From [1].
Antibody information

Company: Abcam

Antibody: Cdk2

Catalog number: ab32147

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Cdk2 aa 250 to the C-terminus (C terminal). Reacts with mouse, rat and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and flow cytometry.

Validation Method

Western blot

Sample

Control and CDK2 ex5(–) K562 cells.

Blocking agent

1% nonfat milk in 1× PBS for 1 h at room temperature.

Primary incubation

For 3 hours.

Secondary incubation

1:5,000 dilution donkey anti-rabbit immunoglobulin G (IgG)-horseradish peroxidase (Amersham).

Detection

ECL (ECL reagents; Boehringer Mannheim).

References
  1. Ji X, Humenik J, Yang D, Liebhaber S. PolyC-binding proteins enhance expression of the CDK2 cell cycle regulatory protein via alternative splicing. Nucleic Acids Res. 2018;46:2030-2044 pubmed publisher