This is a knockout-validated antibody summary, based on the publication "Cyclin-dependent kinase 1 (Cdk1) is essential for cell division and suppression of DNA re-replication but not for liver regeneration", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: Cdk1

Catalog number: sc-54

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 224-230, mapping within a central region of human Cdc2 p34 (Cdk1). Recommended for detection of Cdc2 p34 of mouse, rat, human and Xenopus origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

Western blot

Cdk1FLOX and and Cdk1NULL MEFs. Cells and tissues were lysed in EBN buffer: 80 mM β-glycerophosphate (pH 7.3), 20 mM EGTA, 15 mM MgCl2, 150 mM NaCl, 0.5% Nonidet P-40, 1 mM DTT, and protease inhibitors (20 μg/mL each of leupeptin, chymostatin, and pepstatin; EI8, EI6, and EI10; Chemicon).

Tris-buffered saline with 0.1% Tween 20 and 5% nonfat dry milk (1706404; Bio-Rad).

Overnight at 4 °C

Horseradish peroxidase-conjugated goat anti-mouse (0031432; Pierce).

Enhanced chemiluminescence (NEL105001EA; PerkinElmer).

Please see 4b in the article [1].

Protein was still detected in the KO liver (Authors say the signal was from other cell types). The figure is not a good example of KO validation. Fig 4b is after a IP with Cdk1-deficient MEFs, and looks better.

The same clone (17) is sold as Santa Cruz Biotechnology sc-54, sc-54 AC, sc-54 HRP; Enzo Life Sciences BML-SA119-0100.