This is a knockout-validated antibody summary, based on the publication "Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunofluorescence of Cby1+/+ or Cby1−/− MEFs stained for polyglutamylated tubulin (Glu-tub, green), Cby1 (red), and DNA (DAPI, blue). Scale bars, 10 μm. From [1].

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz Biotechnology

Antibody: Cby1

Catalog number: 8-2

Summary: Mouse monoclonal IgG2a (kappa light chain) raised against amino acids 1-63 of chibby of human origin. Recommended for detection of Chibby of mouse, rat and human origin by western blot and immunoprecipitation.

Immunocytochemistry

Cby1+/+ or Cby1−/− MEFs.

PBS containing 3% bovine serum albumin (Sigma-Aldrich), 0.1% Triton X-100, and 0.02% sodium azide (PBS-BT).

1:100 dilution in PBS-BT for 1–3 h at room temperature or overnight at 4°C.

Alexa Fluor dye–conjugated secondary antibodies (Invitrogen) were diluted in PBS-BT 1:500 and incubated at room temperature for 45 min.

images were acquired using an Axiovert 200M microscope (Carl Zeiss, Jena, Germany) with Plan-Neofluar 100×/1.3 numerical aperture (NA) and PlanApoChromat 63×/1.4 NA objectives and a cooled, charge-coupled device (CCD) camera (Orca ER; Hamamatsu Photonics, Hamamatsu, Japan).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.