Caveolin 3 antibody | knockout validation | BD Biosciences 610421

This is a knockout-validated antibody summary, based on the publication "Caveolin-3 deficiency associated with the dystrophy P104L mutation impairs skeletal muscle mitochondrial form and function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Caveolin 3 antibody | knockout validation | BD Biosciences 610421 figure 1
Figure 1. Crude muscle homogenates prepared from gastrocnemius muscle of three wild type (WT, +/+) and three Cav3 knockout (Cav3KO, −/−) mice were subject to sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotted with antibodies to proteins shown. From [1].
Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: Caveolin 3

Catalog number: 610421

Summary: Mouse monoclonal antibody against rat Caveolin 3 (aa. 3-24). Reacts with rat, mouse and rabbit. Suitable for western blot, immunohistochemistry, and immunofluorescence. Not recommended for immunoprecipitation.

Validation Method

Western blot

Sample

WT and Cav3 knockout mice muscle.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:5,000 dilution horse-radish peroxidase (HRP) conjugated secondary mouse antibodies (Cell Signaling Technology, #7076S).

Detection

Enhanced chemiluminescence (Pierce-Perbio Biotech, Tattenhall, UK) by exposure

to autoradiographic film (Konica Minolta (Tokyo, Japan).

References
  1. Shah D, Nisr R, Stretton C, Krasteva Christ G, Hundal H. Caveolin-3 deficiency associated with the dystrophy P104L mutation impairs skeletal muscle mitochondrial form and function. J Cachexia Sarcopenia Muscle. 2020;11:838-858 pubmed publisher