This is a knockout-validated antibody summary, based on the publication "Glucocorticoid-mediated induction of caveolin-1 disrupts cytoskeletal organization, inhibits cell migration and re-epithelialization of non-healing wounds", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting of the indicated proteins in human keratinocytes expressing endogenous levels of Cav1 (WT) and Cav1-knockout cells (Cav1KO). From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Caveolin-1

Catalog number: 3267

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Glu20 of human caveolin-1. Reacts with human, mouse, rat, hamster, monkey, bovine, and dog. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin and frozen), immunofluorescence/immunocytochemistry and flow cytometry (fixed/permeabilized).

Western blot

WT and Cav1-knockout HaCaT cells.

TBS, 0.1% Tween20, 5% BSA for 1 h.

1:2,000 dilution overnight.

1:1,000 dilution anti-rabbit IgG HRP (Cell Signaling, #7074).